NUKLEONIKA 2006, 51(Suppl. 2):s17-s19

TRITIUM KINETIC EFFECTS ON ENZYMATIC DECOMPOSITION OF L-TRYPTOPHAN

Elżbieta Boroda, Ryszard Kański, Marianna Kańska

Department of Chemistry, University of Warsaw, 1 Pasteura Str., 02-093 Warsaw, Poland

The tritium kinetic isotope effect on position 2 has been determined in the reaction of decomposition of L-tryptophan, L-Trp, catalyzed by enzyme TPase, (EC 4.1.99.1). The numerical values of isotope effects in the course of reaction were obtained by the competitive method using [1-14C]-L-tryptophan as internal radiometric standard.