NUKLEONIKA 2005, 50(3):91-96
A. R. Jalilian1, P. Rowshanfarzad1, K. Shafaii2, M. Kamali-Dehghan1, J. Moafian1, M. Akhlaghi1, M. Babaii3 S. Rajabifar1, M. Mirzaii1
1 Cyclotron and Nuclear Medicine Department, Nuclear Research Center for Agriculture
and Medicine (NRCAM), Atomic Energy Organization of Iran (AEOI),
Karaj, Iran, P. O. Box 31485-498
2 Nuclear Agriculture Department, Nuclear Research Center for Agriculture
and Medicine (NRCAM), Atomic Energy Organization of Iran (AEOI), Karaj, Iran
3 Nuclear Research Center, Atomic Energy Organization of Iran (AEOI),
North-Kargar Str., Tehran, Iran
Human polyclonal antibody (HIgG) was successively labeled with 111In-indium chloride after residulation
with freshly prepared cyclic DTPA-dianhydride. The best results of the conjugation were obtained by the
addition of solid DTPA-dianhydride (0.1-0.3 mg) to 100 ml of the HIgG
solution (0.2-0.4 mg/ml) at pH = 6 in phosphate buffer media at 25°C with continuous stirring for 30 min.
Radio-thin-layer chromatography showed an overall radiochemical yield of 96-99% at optimized conditions
(specific activity = 300-500 MBq/mg, radiochemical purity >98%). The final isotonic 111In-DTPA-HIgG
complex was checked by radio-TLC to ensure the formation of only one species followed by filtration
through a 0.22 m filter. Preliminary long-term in vivo studies in
turpentine-oil induced inflammation in rat model was performed to determine late complex distribution
of the radioimmunoconjugate. The target/skin and target/blood ratios were 27 and 51 after 24 h, and 23
and 51 after 110 h, showing a high selectivity of the radiopharmaceutical for inflammatory lesions.